Pollination failure in mediterranean Ruscus aculeatus L.

Assessment Report on Ruscus Aculeatus L., Rhizoma

In Vivo and in Vitro Comparative Ultrastructural Studies of Ruscus Aculeatus L. Phylloclade Cells

A comparative study of the Ruscus aculeatus phylloclade cells structure and ultrastructure of plants belonging to the native habitat and in vitro culture are presented in the paper in order to establish an adequate protocol for ex situ conservation. In our experimental conditions no anomaly of the phylloclade cells ultrastructure has been observed. Some interesting modifications appeared at the...

Novel steroidal components from the underground parts of Ruscus aculeatus L.

Two new furostanol saponins 1–2 and three new sulphated glycosides 3a,b and 4 were isolated from the underground parts of Ruscus aculeatus L., along with four known furostanol and one spirostanol saponins 5–9 and three free sterols. All of the structures have been elucidated on the basis of spectroscopic data 1D and 2D NMR experiments, MS spectra and GC analyses.

Ex situ conservation of Ruscus aculeatus L. – ruscogenin biosynthesis, genome-size stability and propagation traits of tissue-cultured clones

Ruscus aculeatus L. is a perennial semi-shrub with distinctive leaf-like branches (cladodes). Rhizomes and roots contain steroidal saponins (ruscogenins) that are used in medicine and cosmetics for their anti-inflammatory, venotonic and antihaemorroidal activity. Problematic cultivation of the species causes in many countries unsustainable over-collection from the wild. Tissue culture propagati...

Effect of organogenesis on steroidal saponin biosynthesis in calli cultures of Ruscus aculeatus.

Calli cultures derived from Ruscus aculeatus rhizomes were investigated for their potential to biosynthesize saponins. The capacity of undifferentiated tissues to form steroidal saponins is very limited, but when the calli developed organogenesis, mainly aerial shoots and roots, the saponin production increased significantly. Plantlets regenerated from aerial shoots of Ruscus calli showed a sap...